File:A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv

From Outreach Wiki
Jump to navigation Jump to search

A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv ‎(Ogg Theora video file, length 6.0 s, 512 × 512 pixels, 3.54 Mbps, file size: 2.53 MB)


Wikimedia Commons Logo This free media file is from Wikimedia Commons. Its description page is included below.

Summary

Description
English: Morphological changes and movement of cells in the presence of MVs. Time-lapse observation of a monolayer of Ca9.22 cells was monitored by an optical microscope (Olympus) equipped with a CCD camera (Andor) in the presence of highly purified MVs isolated by the SB technique. The protein concentration of MVs used in the assay was 20 µg/ml. Images were taken at 30 sec/frame for 30 min immediately after addition of MVs suspended in Eagle’s balanced salt solution (EBSS). The constructed time-lapse images are shown at 100 msec/frame (300 times the original speed). The width of the image is 170 µm.
Date
Source Video S1 from Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H (2014). "A Novel Approach for Purification and Selective Capture of Membrane Vesicles of the Periodontopathic Bacterium, Porphyromonas gingivalis: Membrane Vesicles Bind to Magnetic Beads Coated with Epoxy Groups in a Noncovalent, Species-Specific Manner". PLOS ONE. DOI:10.1371/journal.pone.0095137. PMID 24830438. PMC: 4022494.
Author Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

Captions

Add a one-line explanation of what this file represents

Items portrayed in this file

depicts

MIME type

application/ogg

checksum

a5184460c7f9042992574bb28c5698282b1ef24a

determination method: SHA-1

data size

2,657,133 byte

duration

6 second

height

512 pixel

width

512 pixel

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current21:03, 22 May 20146.0 s, 512 × 512 (2.53 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

Metadata

This file contains additional information, probably added from the digital camera or scanner used to create or digitize it.

If the file has been modified from its original state, some details may not fully reflect the modified file.

Retrieved from "https://outreach.wikimedia.org/wiki/File:A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv"