File:Hematopoietic-Stem-Cell-Cytokines-and-Fibroblast-Growth-factor-2-Stimulate-Human-Endothelial-Cell-pone.0085147.s006.ogv

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Hematopoietic-Stem-Cell-Cytokines-and-Fibroblast-Growth-factor-2-Stimulate-Human-Endothelial-Cell-pone.0085147.s006.ogv ‎(Ogg Theora video file, length 20 s, 688 × 595 pixels, 2.68 Mbps, file size: 6.31 MB)


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Summary

Description
English: Real-time video of EC sprouting, tube formation and pericyte recruitment to tubes in 3D fibrin matrices under serum-free defined conditions. ECs were labeled with membrane-targeted GFP and pericytes were labeled with mCherry. Pericytes were mixed in fibrin matrices with added hematopoietic stem cell cytokines and FGF-2. ECs were primed with VEGF/FGF-2 overnight and were seeded on the surface of the fibrin gels. The video shows EC sprouting which in many cases appears directed toward pericytes, EC tube formation, pericyte recruitment to tubes, and pericyte motility along EC-lined tubes from 24-48 hr. The video is shown at 8 frames/sec.
Date
Source Video S6 from Smith A, Bowers S, Stratman A, Davis G (2013). "Hematopoietic Stem Cell Cytokines and Fibroblast Growth factor-2 Stimulate Human Endothelial Cell-Pericyte Tube Co-Assembly in 3D Fibrin Matrices under Serum-Free Defined Conditions". PLOS ONE. DOI:10.1371/journal.pone.0085147. PMID 24391990. PMC: 3877341.
Author Smith A, Bowers S, Stratman A, Davis G
Permission
(Reusing this file)
Creative Commons CC-Zero This file is made available under the Creative Commons CC0 1.0 Universal Public Domain Dedication.
The person who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law. You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission.

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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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WikiProject Open Access

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Date/TimeThumbnailDimensionsUserComment
current13:57, 17 December 201420 s, 688 × 595 (6.31 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

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