File:IRM LDCV fusion after high K.ogv

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IRM_LDCV_fusion_after_high_K.ogv(Ogg Theora video file, length 5.7 s, 112 × 144 pixels, 207 kbps, file size: 144 KB)


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Description
English: Chromaffin cell imaged using interference reflection microscopy. Before stimulation, the footprint is largely dark. When the cell is superfused with a solution containing 60mM potassium, large dense core vesicles begin to fuse with the membrane, appearing as bright spots within the dark footprint.
Date
Source Own work
Author Jhpbroeke

imaged using 427nm light with an image interval of 100ms (10Hz) using a 60x objective (NA 1.45) with an additional 1.6x optovar. Converted to AVI with ImageJ and to OGG with SUPER.

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3 February 2010

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Date/TimeThumbnailDimensionsUserComment
current11:44, 15 February 20105.7 s, 112 × 144 (144 KB)Jhpbroekeencoder problem fixed: height and width are now multiples of 16
11:27, 15 February 20105.7 s, 118 × 146 (137 KB)Jhpbroeke{{Information |Description={{en|1=Chromaffin cell imaged using interference reflection microscopy. Before stimulation, the footprint is largely dark. When the cell is superfused with a solution containing 60mM potassium, large dense core vesicles begin to

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