File:Longitudinal-Intravital-Imaging-of-the-Retina-Reveals-Long-term-Dynamics-of-Immune-Infiltration-and-Video 10.ogv

From Outreach Wiki
Jump to navigation Jump to search

Original file ‎(Ogg Theora video file, length 6.7 s, 994 × 994 pixels, 4.3 Mbps, file size: 3.41 MB)


Wikimedia Commons Logo This free media file is from Wikimedia Commons. Its description page is included below.

Summary

Description
English: Time-lapse intravital imaging of the retina of a healthy CERTN L15 mouse reveals neuronal calcium and, hence, neuronal function is not disturbed by the laser beam during multiphoton microscopy. Three-dimensional 1,400 μm × 1,400 μm × 300 μm FRET ratio images were acquired every minute, over 20 min in the retina of healthy CerTN L15 mice. In this mouse strain, some neuronal subsets express the TN L15 genetically encoded Ca2+ biosensor. The TN L15 biosensor is a FRET-biosensor based on Troponin C, a calcium-sensitive protein. The FRET pair contains Cerulean (cyan fluorescence) as donor and Citrine (yellow fluorescence) as acceptor. We used a three channel-based ratiometric FRET evaluation method to calculate the FRET ratio in the neurons and to evaluate the sustained neuronal calcium concentration. z-step = 6 μm.
Date
Source Video S10 from Bremer D, Pache F, Günther R, Hornow J, Andresen V, Leben R, Mothes R, Zimmermann H, Brandt A, Paul F, Hauser A, Radbruch H, Niesner R (2016). "Longitudinal Intravital Imaging of the Retina Reveals Long-term Dynamics of Immune Infiltration and Its Effects on the Glial Network in Experimental Autoimmune Uveoretinitis, without Evident Signs of Neuronal Dysfunction in the Ganglion Cell Layer". Frontiers in Immunology. DOI:10.3389/fimmu.2016.00642. PMID 28066446. PMC: 5179567.
Author Bremer D, Pache F, Günther R, Hornow J, Andresen V, Leben R, Mothes R, Zimmermann H, Brandt A, Paul F, Hauser A, Radbruch H, Niesner R
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

Captions

Add a one-line explanation of what this file represents

Items portrayed in this file

depicts

inception

23 December 2016

MIME type

application/ogg

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current03:36, 1 February 20176.7 s, 994 × 994 (3.41 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

Metadata

This file contains additional information, probably added from the digital camera or scanner used to create or digitize it.

If the file has been modified from its original state, some details may not fully reflect the modified file.

Retrieved from "https://outreach.wikimedia.org/wiki/File:Longitudinal-Intravital-Imaging-of-the-Retina-Reveals-Long-term-Dynamics-of-Immune-Infiltration-and-Video_10.ogv"