English: Supplementary Movie 2 Network of minor-groove interactions around the 50S E site. Network of minor-groove interactions around the 50S E site. The 23S rRNA helix H68 is at the center of a complex network of minor groove interactions. H68 interacts with the static H75 at all times; however, this minor groove surface of H68 is flanked by two dynamic minor-groove interactions with the acceptor arm of the deacylated tRNA and H76 of the L1 stalk. H68 is also a point of contact with the 30S subunit though intersubunit bridge B7a. Interpolations of the structural transitions through the four functional states were rendered using PyMol28 (PDB IDs - classical state with vacant E site, 4GD21; P/E hybrid state, 4V9023; chimeric pe/E state, 4V9L18 and classical state, with E/E tRNA, 4V6F7.)