File:Transcription-Factor-Mediated-DNA-Looping-Probed-by-High-Resolution-Single-Molecule-Imaging-in-Live-pbio.1001591.s008.ogv

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Transcription-Factor-Mediated-DNA-Looping-Probed-by-High-Resolution-Single-Molecule-Imaging-in-Live-pbio.1001591.s008.ogv(Ogg Theora video file, length 5.0 s, 188 × 174 pixels, 197 kbps, file size: 120 KB)


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Summary

Description
English: Fluorescence movie montage for strain λΔOL corresponding to the data in Figure 2d. Single-color images for TetR-EYFP (top left) and LacI-mCherry (top right) data have intensities scaled linearly from the lowest to the highest pixel values in the first image in each time series. Before creating the overlay images (bottom), single-color images were background subtracted and bandpass filtered using the program ImageJ [91]. The overlay images are scaled to be twice as large as the single-color images. Scale bars correspond 4 µm in the small, single-color images and 2 µm in the overlay image. Ten consecutive image frames are shown in real time (10 frames per second); the movie is looped 5 times.
Date
Source Movie S2 from Hensel Z, Weng X, Lagda A, Xiao J (2013). "Transcription-Factor-Mediated DNA Looping Probed by High-Resolution, Single-Molecule Imaging in Live E. coli Cells". PLOS Biology. DOI:10.1371/journal.pbio.1001591. PMID 23853547. PMC: 3708714.
Author Hensel Z, Weng X, Lagda A, Xiao J
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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June 2013

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Date/TimeThumbnailDimensionsUserComment
current01:05, 2 August 20135.0 s, 188 × 174 (120 KB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

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